8/6/2023 0 Comments Hollow cathode lamp faasThe developed method also provides low usage of sample solution in contrast to conventional nebulizer in the FAAS system.Ĭoncentric nebulizer Flame atomic absorption spectrophotometry High performance liquid chromatography T-shaped slotted quartz tube Vitamin B12.Ĭopyright © 2022. The hollow cathode lamp (HCL) uses a cathode made of the element of interest with a low internal pressure of an inert gas. AAS (Atomic Absorption Spectroscopy) is a quantitative analytical technique used to measure very small concentrations of ions in substances. Two different vitamin tablets were analyzed by the optimized hyphenated system. A successful separation and detection of the analyte was achieved within 3.0 min that offers high sample throughput. Working of Hollow Cathode Lamp How HCL are used Hollow Cathode Lamps are ideal source of spectral lines that are used for testing in laboratories. Recovery studies were also conducted to verify the accuracy and applicability of the developed method for vitamin tablets and excellent percent recovery results (~ 100%) with low standard deviation values were obtained when matrix-matching calibration strategy was performed for each vitamin tablet. Hollow cathode lamps produced by Photron are designed and manufactured to achieve all of the following fundamental requirements for a spectral line source. July 4th, 2018 The idea of this blog post is to cover working aspect of Hollow Cathode Lamps followed by its various advantages that fulfills Hollow Cathode Lamps’ usage in AAS. 1.2 Types The field of atomic absorption spectroscopy (AAS) includes: flame (FAAS) and electrothermal (EAAS or ETAAS) atomic absorption spectroscopy (Skoog et al., 2008). The energy source most frequently used in atomic absorption spectroscopy is the hollow-cathode lamp. Limit of detection (LOD) and limit of quantitation (LOQ) for the HPLC-NI-T-SQT-FAAS system were calculated to be 1.6 and 5.3 mg/kg as Co, respectively. this procedure improves the results obtained with individual lamps. After optimizing some parameters such as mobile phase flow rate and pH, nebulizer gas flow rate, T-SQT height and injection volume, linear range for the analyte was determined between 4.7 and 92 mg/kg as Co. Vitamin B12 was selected as an analyte to show the applicability of the new hyphenated system. There are two reasons for the hollow cathode design. T-SQT was also used to intensify the interaction of atoms with the hollow cathode lamp light. A glass concentric nebulizer was linked to T-shaped slotted quartz tube (T-SQT) using a tubing to transfer the liquid solution eluted from the chromatographic system into the atomization region of FAAS system. A novel nebulizer interface (NI) was proposed to combine high performance liquid chromatography (HPLC) and flame atomic absorption spectrophotometer (FAAS).
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